Chromophore reduction plus reversible photobleaching: how the mKate2 “photoconversion” works
نویسندگان
چکیده
mKate red-to-green photoconversion is a non-canonical type of phototransformation in fluorescent proteins, with poorly understood mechanism. We have hypothesized that the daughter mKate2 protein may also be photoconvertible, and this phenomenon would connected mKate(2) chromophore photoreduction. Indeed, upon intense irradiation sample supplemented by sodium dithionite, accumulation green as well blue spectral forms enhanced. The reaction was shown to reversible reductant’s removal. However, an analysis fluorescence microscopy data, absorption spectra, kinetics time-resolved spectroscopy revealed short-wavelength exist before photoactivation, their fractions increase light-independently after dithionite addition, conversion facilitated photobleaching red form.
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ژورنال
عنوان ژورنال: Photochemical and Photobiological Sciences
سال: 2021
ISSN: ['1474-9092', '1474-905X']
DOI: https://doi.org/10.1007/s43630-021-00060-8