Chromophore reduction plus reversible photobleaching: how the mKate2 “photoconversion” works

نویسندگان

چکیده

mKate red-to-green photoconversion is a non-canonical type of phototransformation in fluorescent proteins, with poorly understood mechanism. We have hypothesized that the daughter mKate2 protein may also be photoconvertible, and this phenomenon would connected mKate(2) chromophore photoreduction. Indeed, upon intense irradiation sample supplemented by sodium dithionite, accumulation green as well blue spectral forms enhanced. The reaction was shown to reversible reductant’s removal. However, an analysis fluorescence microscopy data, absorption spectra, kinetics time-resolved spectroscopy revealed short-wavelength exist before photoactivation, their fractions increase light-independently after dithionite addition, conversion facilitated photobleaching red form.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Primary Role of the Chromophore Bond Length Alternation in Reversible Photoconversion of Red Fluorescence Proteins

Rapid photobleaching of fluorescent proteins can limit their use in imaging applications. The underlying kinetics is multi-exponential and strongly depends on the local chromophore environment. The first, reversible, step may be attributed to a rotation around one of the two exocyclic C-C bonds bridging phenol and imidazolinone groups in the chromophore. However it is not clear how the protein ...

متن کامل

Theoretical study of the reversible photoconversion mechanism in Dronpa

We propose a new scheme of the photoactivation and the photobleaching for Dronpa with molecular dynamics method and density functional theory. These processes can be explained by considering cis-trans isomerization in neutral state of chromophore. The proton transfer from anionic to neutral chromophore makes cis-trans isomerization possible via hula-twist rotation process, since the space for c...

متن کامل

How the system works

My visit at KTH aimed to develop a system in which audio-visual feedback is provided to users who are free to move in the space. The feedback depends on the expressivity of the body movements they perform. Specifically, users can render a music performance by controlling in realtime acoustic parameters through their full-body movement and get a visual feedback on a screen in front of them with ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Photochemical and Photobiological Sciences

سال: 2021

ISSN: ['1474-9092', '1474-905X']

DOI: https://doi.org/10.1007/s43630-021-00060-8